{"id":962,"date":"2019-05-23T17:28:18","date_gmt":"2019-05-23T21:28:18","guid":{"rendered":"https:\/\/www2.whoi.edu\/site\/repetagroup\/?page_id=962"},"modified":"2022-12-23T13:19:44","modified_gmt":"2022-12-23T17:19:44","slug":"thalassiosira-pseudonana-analytical-results","status":"publish","type":"page","link":"https:\/\/www2.whoi.edu\/site\/repetagroup\/specdrom\/phytoplankton-cultures\/thalassiosira-pseudonana\/thalassiosira-pseudonana-analytical-results\/","title":{"rendered":"Thalassiosira pseudonana Analytical Results"},"content":{"rendered":"\n\n\t

Analytical Results<\/h1>\n

Chromatographic Overviews<\/h3>\n

Mass Spectral<\/h3>\n

For each mass spectral acquisition polarity, two visualizations are presented:<\/p>\n

Total Ion Chromatogram (“TIC”)<\/h3>\n

The TIC is a trace of the summed intensity of all ions as a function of time.<\/p>\n

Note that in runs of complex samples such as these algal exudates, there are so many compounds co-eluting that individual peaks are not observed on the trace. Greater information visualization is possible in the Topological Overview.<\/p>\n

A TIC is available for the\u00a0positive<\/a>\u00a0and\u00a0negative<\/a>\u00a0electrospray mode.<\/p>\n

Topological (“Map”)<\/h3>\n

This is a top-down, or bird’s-eye view of the data sets, plotting mass as a function of time, with mass intensity increasing with color intensity.<\/p>\n

A topological view is available for the\u00a0positive<\/a>\u00a0 and\u00a0negative<\/a>\u00a0electrospray mode.<\/p>\n

Optical (UV-Visible)<\/h3>\n

For the optical absobance acquisition, two types of visualization are presented:<\/p>\n

3D<\/h3>\n

These are various views of\u00a0intensity vs. wavelength vs. time<\/a>.<\/p>\n

Specific Wavelength<\/h3>\n

Two generally diagnostic\u00a0wavelengths<\/a>\u00a0(254 and 290 nm) are presented.<\/p>\n

Compound Summary Table<\/h3>\n

A\u00a0table<\/a>\u00a0showing potential retention-time \/ molecular weight pairs, and their molecular ions as determined by the “mzMine” program ( see “http:\/\/mzmine.sourceforge.net” for more information on this program).<\/p>\n\n","protected":false},"excerpt":{"rendered":"

Analytical Results Chromatographic Overviews Mass Spectral For each mass spectral acquisition polarity, two visualizations are presented: Total Ion Chromatogram (“TIC”) The TIC is a trace of the summed intensity of all ions as a function of time. Note that in runs of complex samples such as these algal exudates, there are so many compounds co-eluting…<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":929,"menu_order":3,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www2.whoi.edu\/site\/repetagroup\/wp-json\/wp\/v2\/pages\/962"}],"collection":[{"href":"https:\/\/www2.whoi.edu\/site\/repetagroup\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www2.whoi.edu\/site\/repetagroup\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www2.whoi.edu\/site\/repetagroup\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www2.whoi.edu\/site\/repetagroup\/wp-json\/wp\/v2\/comments?post=962"}],"version-history":[{"count":3,"href":"https:\/\/www2.whoi.edu\/site\/repetagroup\/wp-json\/wp\/v2\/pages\/962\/revisions"}],"predecessor-version":[{"id":1306,"href":"https:\/\/www2.whoi.edu\/site\/repetagroup\/wp-json\/wp\/v2\/pages\/962\/revisions\/1306"}],"up":[{"embeddable":true,"href":"https:\/\/www2.whoi.edu\/site\/repetagroup\/wp-json\/wp\/v2\/pages\/929"}],"wp:attachment":[{"href":"https:\/\/www2.whoi.edu\/site\/repetagroup\/wp-json\/wp\/v2\/media?parent=962"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}