{"id":450,"date":"2020-01-28T11:20:30","date_gmt":"2020-01-28T15:20:30","guid":{"rendered":"https:\/\/www2.whoi.edu\/site\/saito-lab\/?page_id=450"},"modified":"2020-01-28T16:06:02","modified_gmt":"2020-01-28T20:06:02","slug":"woods-hole-proteomics-laboratory","status":"publish","type":"page","link":"https:\/\/www2.whoi.edu\/site\/saito-lab\/woods-hole-proteomics-laboratory\/","title":{"rendered":"Woods Hole Proteomics Laboratory"},"content":{"rendered":"\n\t

Woods Hole Proteomics Laboratory<\/h1>\n

The Saito Laboratory<\/a> operates a proteomics capability for our research group’s microbial and bioinorganic research program as well as being involved in a number of collaborative proteomics studies with colleagues from around the world.<\/p>\n

Our focuses include proteomic profiling to examine changes in protein expression in experimental studies, development of novel metalloprotein discovery, and development of metaproteomic methodologies compatible for autonomous and sectional sampling programs such as GEOTRACES.<\/p>\n

Those interested in potential collaborative projects, contact Dr. Mak Saito or Dr. Matt McIllvin at the Proteomics Laboratory email address: proteomics (at) whoi.edu<\/p>\n

Our proteomics publications are available on the Saito Laboratory<\/a> publications page.<\/p>\n

Support for our research comes from the Gordon and Betty Moore Foundation, the National Science Foundation, and the WHOI Ocean Life Institute.<\/p>\n\t\t\t\t\n\t\t\t\t\"The\n\t\t\t\t<\/a>\n\t

Publications<\/h2>\n\u00bb Saito et al., 2011 PNAS<\/a>
\nSaito, M.A., E.M. Bertrand, V. Bulygin, D. Moran, S. Dutkiewicz, F.M. Monteiro, M.J. Follows, F.W. Valois, J.B. Waterbury. 2011. Iron Conservation by Reduction of Metalloenzyme Inventories in the Marine Diazotroph Crocosphaera watsonii. Proc. Natl. Acad. Sci. doi:10.1073\/pnas.1006943108.\n

\u00bb Bertrand et al., 2011 Env. Microbiol.<\/a><\/p>\n

Erin M. Bertrand, Mak A. Saito, Young Jae Jeon and Brett A. Neilan. 2011., Vitamin B12 biosynthesis gene diversity in the Ross Sea: the identification of a new group of putative polar B12 biosynthesizer, Environmental Microbiology, doi:10.1111\/j.1462-2920.2011.02428.x<\/p>\n\u00bb Saito et al., Frontiers 2011<\/a>
\nMak Saito, Vladimir Bulygin, Dawn Moran, Craig Taylor, and Christopher Scholin. 2011. Examination of Microbial Proteome Preservation Techniques Applicable to Autonomous Environmental Sample Collection. Frontiers in Aquatic Microbiology.\n\u00bb Wurch et al., 2011<\/a>
\nLouie L. Wurch, Erin M. Bertrand, Mak A. Saito, Benjamin A.S. Van Mooy and Sonya T. Dyhrman. 2011. Proteome changes driven by phosphorus stress and recovery in the brown tide-forming alga, Aureococcus anophagefferens. PLoS One.\n\u00bb Dyhrman_PLOSONE2012<\/a>
\nSonya T. Dyhrman, Bethany D. Jenkins, Tatiana A. Rynearson, Mak A. Saito, Melissa L. Mercier, Harriet Alexander, LeAnn P. Whitney, Andrea Drzewianowski, Vladimir V. Bulygin, Erin M. Bertrand, Zhijin Wu, Claudia Benitez-Nelson, Abigail Heithoff. 2012 Coordination in the transcriptome and proteome of the diatom Thalassiosira pseudonana reveals a diverse phosphorus stress response. PLoS One.\n\t

Datasets<\/h2>\n\u00bb PNAS2011Supplemental<\/a>
\nSupplemental Proteomics dataset with spectral counting results and identified tryptic peptides from diel cycle and iron limitation of Crocosphaera watsonii Saito et al., PNAS 2011 (also available on PNAS site as an open access document).
\nhttp:\/\/www.pnas.org\/content\/early\/2011\/01\/14\/1006943108.full.pdf+html\n\u00bb Wurch_TableS1Peptides<\/a>
\nIdentified peptides in Wurch et al., PLOSONE, 2011: Proteome Changes Driven by Phosphorus Deficiency and Recovery in the Brown Tide-Forming Alga Aureococcus anophagefferens
\nhttp:\/\/www.plosone.org\/article\/info%3Adoi%2F10.1371%2Fjournal.pone.0028949\n\u00bb Wurch_TableS2SpectralCounts<\/a>
\nProtein spectral count data and corresponding transcriptome results from Wurch et al., PLOSONE, 2011: Proteome Changes Driven by Phosphorus Deficiency and Recovery in the Brown Tide-Forming Alga Aureococcus anophagefferens
\nhttp:\/\/www.plosone.org\/article\/info%3Adoi%2F10.1371%2Fjournal.pone.0028949\n\u00bb Dyhrman_PLOSONE2012_Table4Peptides<\/a>
\nPeptides from Thalassiosira pseudonana under phosphorus-limited and replete conditions. From Dyhrman et al., PLOSONE, 2012.
\nhttp:\/\/www.plosone.org\/article\/info%3Adoi%2F10.1371%2Fjournal.pone.0033768#s4\n\u00bb Dyhrman_PLOSONE_Table5SupplementalSpectralCounts<\/a>
\nSpectral count and corresponding transcriptome data from phosphorus-limited and replete Thalassiosira pseudonana (marine diatom), Dyhrman et al., 2012 PLOSONE.
\nhttp:\/\/www.plosone.org\/article\/info%3Adoi%2F10.1371%2Fjournal.pone.0033768#s4\n\t\t\t\t\n\t\t\t\t\"Global\n\t\t\t\t<\/a>\n\t\tGlobal proteome analysis of Crocosphaera watsonii (from Saito et al 2011 PNAS)\n\t\t\t\t\n\t\t\t\t\"Global\n\t\t\t\t<\/a>\n\t\tGlobal proteome analysis of Crocosphaera watsonii (from Saito et al 2011 PNAS)\n","protected":false},"excerpt":{"rendered":"

Woods Hole Proteomics Laboratory The Saito Laboratory operates a proteomics capability for our research group’s microbial and bioinorganic research program as well as being involved in a number of collaborative proteomics studies with colleagues from around the world. Our focuses include proteomic profiling to examine changes in protein expression in experimental studies, development of novel…<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www2.whoi.edu\/site\/saito-lab\/wp-json\/wp\/v2\/pages\/450"}],"collection":[{"href":"https:\/\/www2.whoi.edu\/site\/saito-lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www2.whoi.edu\/site\/saito-lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www2.whoi.edu\/site\/saito-lab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www2.whoi.edu\/site\/saito-lab\/wp-json\/wp\/v2\/comments?post=450"}],"version-history":[{"count":3,"href":"https:\/\/www2.whoi.edu\/site\/saito-lab\/wp-json\/wp\/v2\/pages\/450\/revisions"}],"predecessor-version":[{"id":467,"href":"https:\/\/www2.whoi.edu\/site\/saito-lab\/wp-json\/wp\/v2\/pages\/450\/revisions\/467"}],"wp:attachment":[{"href":"https:\/\/www2.whoi.edu\/site\/saito-lab\/wp-json\/wp\/v2\/media?parent=450"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}