Similarly, the studies of novel mechanisms of developmental effects of toxins, which we have uncovered, employ modern technologies, including transgenic zebrafish lines, high-speed video analysis of behavior, and epitranscriptomics.<\/p>\n\t\t\t\t The Environmental Sample Processor (ESP) is a novel robotic system that remotely collects samples of seawater, filters out cells, breaks them apart, and then analyzes the DNA and indentifying chemicals resulting from the cellular decomposition.\u00a0 The data is then transmitted back to scientists in the lab. Moored at sea, the ESP allows for the real time monitoring of ocean conditions, allowing scientists to detect harmful algal blooms (HAB) as they begin.\u00a0 This provides an effective early warning system and can also collect in situ data needed to improve forecast models. Furthermore, the ESP is a versatile system and can be calibrated to detect not only algae but bacterial or larval organisms as well.\u00a0 Ongoing research is also developing capabilities for HAB toxin measurements on the ESP. This makes the ESP an ideal system for a variety of different scientific applications. Developed by the Monterey Bay Aquarium Research Institute and built by McLane Research Laboratories, further information regarding the ESP can be found below.<\/p>\n The Imaging FlowCytobot (IFCB) is a system that utilizes video and flow cytometric technology to photograph and classify a variety of microorganisms, revealing to scientists on shore the growth and decline of diverse plankton populations. The system acts as a type of submerged microscope, analyzing microorganisms on a continuous basis, while simultaneously transmitting this information to the shore station in real time. Capable of long-term deployment, the FlowCytobot offers a comprehensive and efficient monitoring of microorganism populations. Through automated image classification methods, phytoplankton and other microorganisms are identified and counted providing unprecidented detail on complexities of plankton dynamics.<\/p>\n One of the advantages of the zebrafish embryo model is the ability to generate transgenic lines in which specific cell types are labeled with fluorescent markers such as green fluorescent protein (GFP; which was first cloned by WHOI researcher Doug Prasher<\/a>). We are using a variety of transgenic lines in which neurons and glial cells (including myelinating oligodendrocytes) are labeled with fluorescent tags. With these lines, we use image analysis and time-lapse imaging in vivo to determine the effects of exposure to toxins such as domoic acid or saxitoxin, or toxicants such as PCBs, on the developmental trajectory and function of these cells. For examples, please see the papers authored by Panlilio et al (2020, 2021, 2023) in the publications list<\/a>.<\/p>\n\t\t\t\t Startle responses in teleosts are elicited by sudden stimuli. Auditory\/vibrational stimuli can lead to one of two types of startle responses – a short-latency c-type (SLC) startle response (<14 ms) or a long-latency c-type (LLC) startle response (>14 ms). These two types of startle response have different latencies, distinct kinematics, and are driven by separate underlying neural\u00a0circuits. The startle response circuits are well known in zebrafish, making it possible to link startle behavioral data to underlying structural and cellular targets (see Transgenic zebrafish<\/em>, above). High-speed videography is required to differentiate these two types of startle responses. See Panlilio et al (2020, 2021) and Brun et al (2021)<\/a> for details of the assay and its use to examine mechanisms of developmental neurotoxicity.<\/p>\n\n","protected":false},"excerpt":{"rendered":" Innovation The Woods Hole COHH is innovative in the research proposed, the technologies employed, our current findings that have led to the research, the broad and perhaps global implications for HAB biology, the models to predict HAB events in a changing ocean, and the new understanding and implications of developmental neurotoxic mechanisms we are uncovering.…<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":4,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www2.whoi.edu\/site\/whcohh\/wp-json\/wp\/v2\/pages\/88"}],"collection":[{"href":"https:\/\/www2.whoi.edu\/site\/whcohh\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www2.whoi.edu\/site\/whcohh\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www2.whoi.edu\/site\/whcohh\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www2.whoi.edu\/site\/whcohh\/wp-json\/wp\/v2\/comments?post=88"}],"version-history":[{"count":3,"href":"https:\/\/www2.whoi.edu\/site\/whcohh\/wp-json\/wp\/v2\/pages\/88\/revisions"}],"predecessor-version":[{"id":1188,"href":"https:\/\/www2.whoi.edu\/site\/whcohh\/wp-json\/wp\/v2\/pages\/88\/revisions\/1188"}],"wp:attachment":[{"href":"https:\/\/www2.whoi.edu\/site\/whcohh\/wp-json\/wp\/v2\/media?parent=88"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}![\"graphics-ESP_dock_test-DSC_8590_151795_259993.jpg\"](\"https:\/\/www2.whoi.edu\/site\/whcohh\/wp-content\/uploads\/sites\/30\/2018\/02\/graphics-ESP_dock_test-DSC_8590_151795_259993.jpg\" "\\"graphics-ESP_dock_test-DSC_8590_151795_259993.jpg\\"")
\n\t\tSenior engineering assistant Will Ostrom guides an Environmental Sample Processor<\/a> (ESP) into the test well at the WHOI dock. The ESP is a seagoing lab<\/a>: it autonomously samples seawater, counts the cells in it, analyzes their DNA and other key chemicals, and relays the data back to scientists on shore. The ESP was developed by WHOI\/MIT Joint Program graduate Chris Scholin<\/a>. (Photo by Jayne Doucette, Woods Hole Oceanographic Institution)\n\tEnvironmental Sample Processor (ESP)<\/h2>\n
Related Links<\/h3>\n
\n
\nOceanus<\/em> Magazine<\/li>\n
\nMcLane Research Laboratories<\/li>\n
\nMonterey Bay Aquarium Research Institute<\/li>\n
\nWoods Hole Oceanographic Instituion<\/li>\n
\nNational Oceanic and Atmospheric Administration<\/li>\n<\/ul>\n\t\t\t\t![\"Imaging_FlowCytobot_en_204353_259893.jpg\"](\"https:\/\/www2.whoi.edu\/site\/whcohh\/wp-content\/uploads\/sites\/30\/2018\/02\/Imaging_FlowCytobot_en_204353_259893.jpg\" "\\"Imaging_FlowCytobot_en_204353_259893.jpg\\"")
\n\t\tImaging FlowCytobot (IFCB). \n\t\t\t\t![\"Photo](\"https:\/\/www2.whoi.edu\/site\/whcohh\/wp-content\/uploads\/sites\/30\/2018\/09\/G0071257.jpg\" "\\"DCIM101GOPRO\\"")
\n\t\tIFCB-ESP co-deployment in Salt Pond. (Photo by Natalie Kukshtel, Northeastern University and Woods Hole Oceanographic Institution)\n\tImaging FlowCytobot (IFCB)<\/h2>\n
Related Links<\/h3>\n
\n
\nOceanus<\/em> Magazine<\/li>\n
\nWoods Hole Oceanographic Institution<\/li>\n
\nWoods Hole Oceanographic Institution<\/li>\n
\nMcLane Research Laboratories<\/li>\n<\/ul>\n\t\t\t\t![\"Panlilio](\"https:\/\/www2.whoi.edu\/site\/whcohh\/wp-content\/uploads\/sites\/30\/2023\/03\/P3_Fig4.png\" "\\"P3_Fig4\\"")
\n\t\tPanlilio JM, Hammar KM, Aluru N, Hahn ME (2023) Developmental exposure to domoic acid targets reticulospinal neurons and leads to aberrant myelination in the spinal cord. Scientific reports. 13(1):2587. https:\/\/doi.org\/10.1038\/s41598-023-28166-2\n\tTransgenic zebrafish<\/h2>\n
![\"Panlilio](\"https:\/\/www2.whoi.edu\/site\/whcohh\/wp-content\/uploads\/sites\/30\/2023\/03\/ehp6652_f1.jpg\" "\\"ehp6652_f1\\"")
\n\t\tPanlilio JM, Aluru N, Hahn ME (2020) Developmental Neurotoxicity of the Harmful Algal Bloom Toxin Domoic Acid: Cellular and Molecular Mechanisms Underlying Altered Behavior in the Zebrafish Model. Environ Health Perspect 128: 117002. https:\/\/ehp.niehs.nih.gov\/doi\/10.1289\/EHP6652\n\tHigh-speed video analysis of startle behavior<\/strong><\/h2>\n