Skip to content

Comparative biology and evolution of the aryl hydrocarbon receptor (AHR)

Halogenated aromatic hydrocarbons (HAHs) and polynuclear aromatic hydrocarbons (PAHs) are ubiquitous environmental contaminants implicated in adverse effects on human health. These compounds cause toxicity by activating the aryl hydrocarbon receptor (AHR), a ligand-activated transcription factor. The AHR is part of an metabolic system that evolved to detect aromatic hydrocarbons and initiate an adaptive response, but it also has physiological roles regulating vascular development, immune function, and cell growth, suggesting possible mechanisms by which chemical exposure could cause toxicity.

To understand these diverse functions and the possible role of AHR in mediating toxicity, we have studied the biology of the AHR in diverse species. We’ve found that vertebrate animals are characterized by the expansion and diversification of AHRs, via gene and genome duplications, from the ancestral protoAHR into at least five classes of AHR-like proteins: AHR, AHR1, AHR2, AHR3, and AHRR. The evolution of multiple AHRs in vertebrates coincided with the acquisition of high-affinity binding of halogenated and polynuclear aromatic hydrocarbons and the emergence of adaptive functions involving regulation of xenobiotic-metabolizing enzymes and roles in adaptive immunity. The existence of multiple AHRs may have facilitated subfunction partitioning and specialization of specific AHR types in some taxa. Additional research in diverse model and non-model species will continue to enrich our understanding of AHR and its pleiotropic roles in biology and toxicology.

Recent papers:

Hahn ME, Karchner SI, Merson RR (2017) Diversity as Opportunity: Insights from 600 Million Years of AHR Evolution. Current Opinion in Toxicology 2: 58-73. PMCID: PMC5343764

Avilla MN, Malecki KMC, Hahn ME, Wilson RH, Bradfield CA (2020) The Ah Receptor: Adaptive Metabolism, Ligand Diversity, and the Xenokine Model. Chemical Research in Toxicology 33:860−879. (https://doi.org/10.1021/acs.chemrestox.9b00476) (PMCID: PMC7175458)

Shankar, P., Dasgupta, S., Hahn, M. E.and Tanguay, R. L. (2020). A Review of the Functional Roles of the Zebrafish Aryl Hydrocarbon Receptors. Toxicological Sciences, 178(2):215-238.(https://doi.org/10.1093/toxsci/kfaa143) (PMCID: PMC7706399)

The aryl hydrocarbon receptor (AHR) pathway in fish

Most fish species have four AHR genes: AHR1a, AHR1b, AHR2a, and AHR2b.

Models for AHR research

We study AHR in a variety of species.

 

AHR Evolution

The evolutionary history of AHR genes, as inferred from studies in extant species, is shown in this figure.

 

Funding Agencies

This research has been supported for many years by the National Institute of Environmental Health Sciences.


Partners/Collaborators

The research has been performed in collaboration with many colleagues. See publication lists for their names.

Related Links and File

Some popular articles

Research Papers

A few of the key papers resulting from this work are listed below.